The researchers have found that there are two layers of characteristic skin cells in Chameleons. The upper layer is more ponderous in males than in females and juveniles, can modulation color by modifying its structure and how it repercussion lights, they said.
Many other animals that switch colors, such as the squid and octopus, chameleons do negative decrease their pigmentation by gathering or disbanding pigments within their skin cells, the researchers found. The researchers also said that the lizards rely on constitutional changes that affect how light reflects off their skin.
Five grown-up males, four developed females and four immature panther chameleons (Furcifer pardalis), a species of lizard that lives in Madagascar were studied to see how the serpents change color. The investigators have found that the chameleons have two superposed opaque layers of iridophore cells, the multicolored cells that have orpiment that reflects light.
The researchers said the key to the chameleons’ dramatic color shifts is that the iridophore cells contain nanocrystals of different sizes, shapes, and combinations. The chameleons can change the skeletal composition of the upper cell layer by loosening or stimulating the skin, which leads to a change in color. For occurrence, a male chameleon might be in an informal state when it’s lynching out on a branch and in an agitated state when it sees a battling male.

Senior author Michel Milankovitch, a savant of genetics and evolution at the University of Geneva in Switzerland says when the skin is in the relaxed state, the nanocrystals in the iridophore cells are very close to each other, and hence the cells specifically reveal short wavelengths, such as blue.
Milankovitch reported to Live Science in an email saying “when the skin converts excited, the league amidst neighboring nanocrystals developments and each iridophore cell selectively muse longer wavelengths, such as yellow, orange or red”. Milankovitch said the lizards‘ skin also includes yellow pigments, and blue mixed with yellow produces green, a cryptic color that conceals them between trees and plants.
The male chameleon adjusts colors from green to yellow. The red skin shade does not change strenuously during incitement, but its illumination increases. The researchers also identified a deeper and thicker layer of skin cells that reflects a huge amount of near-infrared light. The researchers said these cells do not develop to change color; it’s probable that they help the chameleons bounce heat and stay cool.
The researchers studied and used a multitude of methods to survey the iridophore cells. They also recorded the chameleon’s color-changing using high-resolution videography and numerical models that prognosticate how the nanocrystals should reflect light.
Milinkovitch said the results are a comprehensive match with what we recognize in real life. The researchers also handled the cells by suppressing them to resolutions of undulating consistencies, which made the cells to expand or shrivel. Just as the researchers prophesied, these adjustments changed the distances between the nanocrystals and altered their visible colors.
Milinkovitch says, only the adult male chameleon’s changes color, especially when they see a rival male chameleon they want to chase away or a female to attract. He said females and young chameleons are dull-colored and have a much reduced upper layer of iridophore cells.
The findings may help physicists and engineers to replicate the chameleon’s color-changing capacities in new technology, such as appliances that eliminate reflection.